Current understanding concerning intestinal stem cells

Table 4 Two systems for expanding intestinal stem cells

Established by	Published year	System type	Substrate	Supplemental factor	Medium	Cultured content	Expanding duration of cultured content
Sato et al[23]	2009	3D	Matrigel containing laminin α1 and α2	N2, B27, Wnt3, EGF, Noggin, R-spondin1, Jagged-1 and Y-27632 dihydrochloride	Serum-free	ISCs	Up to 1 yr
Ootani et al[62]	2009	3D	Collagen gel containing collagen I and III	/	Containing 20% of FCS in medium	Small pieces of intestinal tissue	Up to 1 yr

EGF: Epithermal growth factor; ISCs: Intestinal stem cells.