Differential diagnosis of gallstones by using hypericin as a fluorescent optical imaging agent

Figure 5 Macroscopic digital imaging under white and UV (365 nm) lights of human cholesterol gallstones treated with different concentrations of hypericin (2 × 10^-5 - 2 × 10^-3 M) or only with solvent for 72 h. Solvent-treated stone (A1, A2) lacked fluorescence (A3, A4). Gallstones pre-incubated with hypericin solutions at 2 × 10^-5 M (B1, B2), 2 × 10^-4 M (C1, C2) and 2 × 10^-3 M (D1, D2) exhibited fluorescence (B4, C4, D4), which seemed to increase with the hypericin concentration (B3, C3, D3).