Glucose deprivation induces chemoresistance in colorectal cancer cells by increasing ATF4 expression

Figure 1 Glucose deprivation promotes drug resistance of colorectal cancer cells. A: GD decreased drug susceptibility to CRC cells. LoVo cells were treated with the indicated doses of the different drugs for 48 h under GD or normal culture condition. The in vitro drug sensitivity was tested by the CCK-8 assay; B: GD inhibited LOHP- and 5-FU-induced apoptosis. LoVo cells were treated with 0.1 μg/mL LOHP or 0.05 μg/mL 5-FU for 48 h. Hoechst 33258 nuclear staining and Annexin V/7-AAD staining assays were performed to detect apoptosis. C and D: GD promoted the expression of resistance gene MDR1. The mRNA and protein levels of MDR1 were examined by qRT-PCR and Western blot, respectively. ^aP < 0.05, ^bP < 0.01,^cP < 0.001, control vs GD. GD: Glucose deprivation; CRC: Colorectal cancer.