Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells

Figure 5 Effects of silybin on oxidative stress markers. In control and steatotic FaO cells incubated in the absence or in the presence of silybin 50 μmol/L we assessed. A: The intracellular level of MDA (pmol MDA/mL x mg of sample protein) quantified by TBARS assay data are expressed as percentage values with respect to controls and normalized for total proteins; B: Catalase specific activity (micromoles of decomposed H₂O₂ per min/mg of sample protein) evaluated by spectrophotometric assay, data are expressed as percentage values with respect to controls and normalized for total proteins. C: Densitometric analysis of nuclear NF-κB/p65 evaluated by Western blotting; β-actin was the protein loading control used as housekeeping gene for normalization; data are expressed as percentage values with respect to controls. Values are mean ± SD from at least three independent experiments. ANOVA followed by Tukey’s test was used to assess the statistical significance between groups Significant differences are denoted by symbols: ^aP≤ 0.001, ^cP≤ 0.01 C vs OP and ^bP≤ 0.001; ^dP≤ 0.01; ^fP≤ 0.05 OP vs silybin.