Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells

Figure 4 Effects of silybin on reactive oxygen species production. The intracellular level of reactive oxygen species (ROS), mainly hydrogen peroxide, was visualized in situ by optical microscopy in control (A and inset) and steatotic FaO cells incubated in the absence (C and D) or in the presence (E and F) of silybin 50 μmol/L. The ROS level were also quantified by spectrofluorimeter assay of DCF-stained cells (B) and data are expressed as percent mean fluorescence intensity (MFI) relative to steatotic cells and normalized for total proteins. DCF: 2’-7’ dichlorofluorescein. ^bP < 0.001 SC vs SC + S