Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells

Figure 3 Effects of silybin on lipid catabolism pathways. In control (C) and steatotic FaO cells incubated in the absence (SC) or in the presence (SC + S) of silybin 50 μmol/L we assessed: mRNA expression of CPT-1 (A) and of Atg7 (B) by qPCR; GAPDH was used as the internal control for quantifying gene expression and data expressed as fold induction with respect to controls; extracellular TG content quantified in the medium by spectrophotometric assay (C); data are expressed as percent TG content relative to control and normalized for total proteins; Values are mean ± SD from at least three independent experiments. ANOVA followed by Tukey’s test was used to assess the statistical significance between groups Significant differences are denoted by symbols: ^aP≤ 0.001, ^eP≤ 0.05 C vs OP and ^bP≤ 0.001, ^dP≤ 0.01 OP vs Slybin.