Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells

Figure 2 Effects of silybin on transcription factors regulating lipid metabolism. In control (C) and steatotic FaO cells incubated in the absence (SC) or in the presence (SC + S) of silybin 50 μmol/L we assessed. A: mRNA expression of PPARα, PPARδ and PPARγ by qPCR; GAPDH was used as the internal control for quantifying gene expression; data expressed as fold induction with respect to controls; B: Densitometric analysis of SREBP-1c by Western blotting; β-actin was the protein loading control used as housekeeping gene for normalization; data are expressed as percentage values with respect to controls. Values are mean ± SD from at least three independent experiments. ANOVA followed by Tukey’s test was used to assess the statistical significance between groups Significant differences are denoted by symbols: ^cP≤ 0.01, ^eP≤ 0.05 C vs OP and ^dP≤ 0.01, ^fP≤ 0.05 OP vs Slybin.