Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori

Table 3 Results for the 23SrRNA point mutation in gastric mucosa and saliva samples of 99 RUT-positive clinical cases detected by nested-ASP-PCR

	RUT-positive	H. pylori-positive detection rate	WT	Total mutation, A-to-G	2142 G	2143 G	2144 G
Gastric mucosa	99	87	30	57	50	52	44
ratio		87.88%	34.48%	65.52%	57.47%	59.77%	50.57%
		(87/99)	(30/87)	(57/87)	(50/87)	(52/87)	(44/87)
Saliva	99	67	22	45	35	39	23
ratio		67.68%	32.84%	67.16%	52.24%	58.21%	34.34%
		(67/99)	(22/67)	(45/67)	(35/67)	(39/67)	(22/67)

H. pylori: Helicobacter pylori; ASP-PCR: Allele-specific primer-polymerase chain reaction.