Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori

Table 2 Results for the 23SrRNA point mutation in 30 gastric mucosa and saliva samples, as detected by ASP-PCR and nested-ASP-PCR

	H. pylori-positive detection rate	WT	Total mutation, A-to-G	2142 G	2143 G	2144 G
Gastric mucosa
ASP-PCR	19	8	11	10	10	10
Ratio	63%	42.11%	57.89	52.63%	52.63%	52.63%
	(19/30)	(8/30)	(11/19)	(10/19)	(10/19)	(10/19)
Nested-ASP-PCR	27	16	11	11	10	11
Ratio	90%	59.26%	40.74%	40.74%	37.04%	40.74%
	(27/30)	(16/30)	(11/19)	(11/19)	(10/19)	(10/19)
Saliva
ASP-PCR	17	7	10	10	4	0
Ratio	56.67%	41.18%	58.82%	58.82%	23.53%	0
	(17/30)	(7/30)	(10/17)	(10/17)	(4/17)
Nested-ASP-PCR	25	9	16	16	13	9
Ratio	83.33%	36%	64%	64%	52%	36%
	(25/30)	(9/30)	(16/25)	(16/25)	(13/25)	(9/25)

ASP-PCR: Allele-specific primer-polymerase chain reaction.