Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori

doi:10.3748/wjg.v22.i25.5822

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 7, 2016; 22(25): 5822-5830
Published online Jul 7, 2016. doi: 10.3748/wjg.v22.i25.5822

Table 1 Primer sequences for the Helicobacter pylori 23SrRNA gene

Gene name	Primer sequence, 5’-3’	Product length (bp)
External-F	GCGTTGAATTGAAGCCCGAGTAAAC	505
External-R	CCGACTTTCGTCTCTGCTTGA
WT2142-F	TCCTACCCGCGGCAAGACGGA	294
MUT2142-F	TCCTACCCGCGGCAAGACGGG
WT2143-F	CCTACCCGCGGCAAGACGGAA
MUT2143-F	CCTACCCGCGGCAAGACGGAG
WT2144-F	CCTACCCGCGGCAAGACGGAAA
MUT2144-F	CCTACCCGCGGCAAGACGGAAG
Inner-R	GCCATTACACTCAACTTGCGATTTC

The underlined primer is designed to match the single nucleotide polymorphism site. WT: Wild-type; MUT: Mutation; F: Forward primer; R: Reverse primer.

Citation: Luo XF, Jiao JH, Zhang WY, Pu HM, Qu BJ, Yang BY, Hou M, Ji MJ. Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori. World J Gastroenterol 2016; 22(25): 5822-5830
URL: https://www.wjgnet.com/1007-9327/full/v22/i25/5822.htm
DOI: https://dx.doi.org/10.3748/wjg.v22.i25.5822