Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Figure 5 TRPV4 activator-induced ATP release in the RGE1-01 cells. A: GSK1016790A (GSK, 100 nmol/L) or 5,6-EET (500 nmol/L) induced significantly higher ATP release in RGE1-01 cells (^aP < 0.05 vs Control). B: Mechanical stimuli were quantitatively applied with a stretch apparatus. Microscopy images demonstrated that cells were stretched laterally without detachment. A 120% stretch induced significantly higher amounts of ATP release from RGE1-01 cells [^cP < 0.05 vs 0% stretch (control)] that could be inhibited by pre-treatment with specific TRPV4 antagonist HC 067047 (1 μmol/L). TRPV4: Transient receptor potential vanilloid 4.