Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Figure 4 TRPV4-mediated current responses in mouse primary gastric epithelial cells and RGE1-01 cells. A: GSK (300 nmol/L) evoked inward current responses in WT primary gastric epithelial cells. Currents in response to ramp-pulses at points a, b and c (left in panel B) are shown (middle), with a strongly outwardly rectifying current-voltage relationship. Significantly larger inward currents at -60 mV were obtained from WT cells (means ± SEM; 0.76 ± 0.27 nA, n = 5) than in TRPV4KO cells (0.01 ± 0.00 nA, n = 5) (^aP < 0.05 vs WT). B: Similar current responses were also obtained in RGE1-01 cells.