Tumor-specific expression of shVEGF and suicide gene as a novel strategy for esophageal cancer therapy

Figure 6 Immunohistochemistry analysis for yCDglyTK and vascular endothelial growth factor in EC9706 xenograft sections. A: Histological expression and distribution of yCDglyTK at magnification × 200; no-treatment control group (a); CPNPs/null + 5-FC (b); CPNPs/shVEGFX (c); CPNPs/yCDglyTK+5-FC (d); CPNPs/shVEGF-yCDglyTK +5-FC (e); B: Integrated optical density (IOD) values of VEGF expression in EC9706 xenografts. Anti-VEGF antibody was used for immunohistochemistry assay; C: Quantification of angiogenesis by microvessel counts (MVC) in EC9706 xenografts. Anti-CD34 was used for microvessel staining. CPNPs: Calcium phosphate nanoparticles; VEGF: Vascular endothelial growth factor; 5-FC: 5-fluorocytosine.