Tumor-specific expression of shVEGF and suicide gene as a novel strategy for esophageal cancer therapy

Figure 3 Changes of vascular endothelial growth factor and yCDglyTK expression in established stable cell lines. A: Representative VEGF mRNA and protein expression were analyzed by RT-PCR (top panel) and western blot (bottom panel), respectively. GAPDH was used as an internal control. Lane 1, EC9706/null; lane 2, EC9706/yCDglyTK; lane 3, EC9706/shVEGF; lane 4, EC9706/shVEGF-yCDglyTK; B: Representative yCDglyTK mRNA and protein expression were analyzed by semiquantitative RT-PCR (top panel) and western blot (bottom panel), respectively. GAPDH was used as an internal control. Lane 1, EC9706/null; lane 2, EC9706/shVEGF; lane 3, EC9706/yCDglyTK; lane 4, EC9706/shVEGF-yCDglyTK. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; RT-PCR: Reverse transcription polymerase chain reaction; VEGF: Vascular endothelial growth factor.