miR-106b promotes cancer progression in hepatitis B virus-associated hepatocellular carcinoma

Figure 5 The mRNA expression levels of miR-106b-25 cluster and MCM7 in hepatitis B virus X protein-transfected hepatocellular carcinoma cell lines (n = 3). The hepatitis B virus X protein (HBx) protein expression plasmid was transiently transfected into Huh7 and Hep-3B cells. miR-106b, miR-93, miR-25, and MCM7 expression levels at 0 (control), 6, 12, and 24 h post transfection were detected using q-RT-PCR. A: miRNAs expression levels in HBx-transfected Huh7 cells; B: miRNAs expression levels in HBx-transfected Hep-3B cells; C: MCM7 expression levels in HBx-transfected Huh7 cells; D: MCM7 expression levels in HBx-transfected Hep-3B cells. Data represent the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^dP < 0.001 vs control. a.u.: Arbitrary unit. HCC: Hepatocellular carcinoma.