Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation

doi:10.3748/wjg.v22.i22.5201

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 14, 2016; 22(22): 5201-5210
Published online Jun 14, 2016. doi: 10.3748/wjg.v22.i22.5201

Table 1 Polymerase chain reaction primers gene sequences

Target gene	Primer sequence	Product length (bp)
ROR-γt	forward: GACGGCCAACTTACTCTTGG	109
ROR-γt	reverse: AGAAACTGGGAATGCAGTGG	109
IL-17A	forward: TCCCTCTGTGATCTGGGAAG	154
IL-17A	reverse: CTCGACCCTGAAAGTGAAGG	154
IL-6	forward:CGGAGAGGAGACTTCACAGAG	105
IL-6	reverse: CATTTCCACGATTTCCCAGA	105
GAPDH	forward: CATGGCCTTCCGTGTTCCTA	83
GAPDH	reverse:TGTCATCATACTTGGCAGGTTTCT	83

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; IL: Interleukin; ROR-γt: Related orphan receptor-γt.

Citation: Huang XL, Zhang X, Fei XY, Chen ZG, Hao YP, Zhang S, Zhang MM, Yu YQ, Yu CG. Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation. World J Gastroenterol 2016; 22(22): 5201-5210
URL: https://www.wjgnet.com/1007-9327/full/v22/i22/5201.htm
DOI: https://dx.doi.org/10.3748/wjg.v22.i22.5201