Hepatocyte transplantation program: Lessons learned and future strategies

Figure 5 Hepatocyte isolation and transplantation process. A: Perfusion of the liver at 37 °C; B: Liver after digestion by collagenase perfusion; C: Purification of hepatocytes by low-speed centrifugation; D and E: Hepatocyte suspension is washed twice and pelleted by low-speed centrifugation; F: Freshly isolated hepatocyte suspension; G: Cell thawing by immersing the bags in a fixed-temperature bath at 37 °C; H: Hepatocyte suspension in infusion medium ready to be transplanted; I: Infusion of hepatocytes to the receptor patient.