Contribution of mammalian target of rapamycin in the pathophysiology of cirrhotic cardiomyopathy

Figure 5 Western blot analysis of p-mammalian target of rapamycin protein in the left ventricles of control and CCl₄-induced cirrhotic rats treated with normal saline or rapamycin (2 mg/kg). The upper panels demonstrate the representative immunoblots of p-mTOR and glyceraldehyde 3 phosphate dehydrogenase (GAPDH) proteins in the control, control + rapamycin, cirrhotic and cirrhotic + rapamycin. The lower panel shows the densitometric analysis after normalization with GAPDH. Values are expressed as p-mTOR/GAPDH ratio (%) and normalized to the control group receiving normal saline (mean ± SD). ^aP < 0.05 vs the other three groups; NS: Non-significant.