Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung’s-associated enterocolitis

Figure 4 Confocal micrograph series. Confocal micrograph series demonstrating the cholinergic nature of TPH2 immuno-positive neurons in the myenteric plexus (A), with ChAT-TPH2 co-expression (white arrow). There was also co-expression of nNOS and TPH2 in the myenteric plexus (B) (white arrow) and submucosal plexus (not shown). VIPergic neurons did not express TPH2 in the myenteric plexus (C). Image (D) shows ChAT immune-positive cholinergic nerve fibres in the circular muscle layer, co-expressing TPH2. TPH2: Tryptophan hydroxylase-2.