Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung&rsquo;s-associated enterocolitis

doi:10.3748/wjg.v22.i19.4662

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 21, 2016; 22(19): 4662-4672
Published online May 21, 2016. doi: 10.3748/wjg.v22.i19.4662

Table 1 Details pertaining to primary and secondary antibodies used in immunofluorescence and Western blot

Host species	Monoclonal/Polyclonal	Antigen	Product code	Manufacturer	Dilution
Rabbit	Polyclonal	Tryptophan hydroxylase 2	NB-100-74555	Novus biologicals (Cambridge, United Kingdom)	1:100
Mouse	Monoclonal	TMEM-16A (ANO1)	Ab190721	Abcam (Cambridge, United Kingdom)	1:200
Mouse	Monoclonal	HuD	sc-48421	Santa-Cruz Biotechnologies (Heidelberg, Germany)	1:100
Mouse	Monoclonal	Platelet-derived growth factor alpha	sc-21789	Santa-Cruz Biotechnologies (Heidelberg, Germany)	1:100
Goat	Polyclonal	Neuronal nitric oxide synthase	ab1376	Abcam (Cambridge, United Kingdom)	1:300
Mouse	Monoclonal	Vasoactive intestinal peptide	sc-25347	Santa-Cruz Biotechnologies (Heidelberg, Germany)	1:100
Mouse	Monoclonal	Choline acetyltransferase	ab49382	Abcam (Cambridge, United Kingdom)	1:100
Mouse	Monoclonal	GAPDH	ab9484	Abcam (Cambridge, United Kingdom)	1:2000
Rabbit	Polyclonal	Mouse (secondary)	ab6728	Abcam (Cambridge, United Kingdom)	1:10000
Donkey	Polyclonal	Rabbit (secondary)	ab6802	Abcam (Cambridge, United Kingdom)	1:10000
Donkey	Polyclonal	Rabbit (Alexa Fluor^® 488)	ab150073	Abcam (Cambridge, United Kingdom)	1:500
Goat	Polyclonal	Mouse (Alexa Fluor^® 594)	ab150116	Abcam (Cambridge, United Kingdom)	1:500
Donkey	Polyclonal	Goat (Alexa Fluor^® 555)	ab150134	Abcam (Cambridge, United Kingdom)	1:500

Citation: Coyle D, Murphy JM, Doyle B, O’Donnell AM, Gillick J, Puri P. Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung’s-associated enterocolitis. World J Gastroenterol 2016; 22(19): 4662-4672
URL: https://www.wjgnet.com/1007-9327/full/v22/i19/4662.htm
DOI: https://dx.doi.org/10.3748/wjg.v22.i19.4662