miR-30b inhibits autophagy to alleviate hepatic ischemia-reperfusion injury via decreasing the Atg12-Atg5 conjugate

Figure 2 miR-30b can alleviate mouse hepatic ischemia-reperfusion injury. A: Hematoxylin and eosin (HE) stain was used to observe histopathologic changes in mouse liver (magnification × 200) after tail intravenous injection of miR-30b-5p agomir or antagomir at 12 h following reperfusion; and B: serum AST and ALT levels; C: Cell apoptosis as measured by terminal uridine nick end labeling (TUNEL). Representative sections as determined at 12 h post-reperfusion (magnification × 200); D: Immunohistochemistry revealed expression of proliferating cell nuclear antigen (PCNA) and caspase-3 (magnification × 200); E: Western blot was used to detect expression of caspase-3, cleave caspase-3, and poly ADP-ribose polymerase 1 (PARP1) in livers, ^aP < 0.05,^bP < 0.01 vs miR-NC group. Every experiment was repeated three times.