miR-30b inhibits autophagy to alleviate hepatic ischemia-reperfusion injury via decreasing the Atg12-Atg5 conjugate

Figure 1 Alterations of miR-30b and autophagy in mouse livers in response to ischemia-reperfusion injury. A: The expression of miR-30b in mouse livers subjected to IR as determined by quantitative real time polymerase chain reaction analysis; B: Western blotting for autophagy-related gene (Atg) 12, light chain 3 (LC3), and cleave caspase-3 in mouse liver; C: IR treatment increases the histopathologic changes in liver (magnification × 200); D: IR treatment increased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in mice compared with sham; E: Transmission electron microscopy (TEM) images of mouse hepatocytes after ischemia followed by reperfusion at 12 h. Scale bars = 2.0 μm. The data were quantified by counting the number of autophagosomes per cross-sectioned cell. ^aP < 0.05,^bP < 0.01,^cP < 0.001 vs Sham group. Every experiment was repeated three times.