Epithelial-to-mesenchymal transition in pancreatic ductal adenocarcinoma: Characterization in a 3D-cell culture model

Figure 9 Podoplanin expression and actin cytoskeleton. A: Micrographs with a confocal microscope of PDAC cells grown in 2D-monolayers and 3D-spheroids, showing the expression of podoplanin. Punctuate immunoreactivity is located in the cytoplasm. Original magnification: 60 ×; B: HPAF-II 3D-spheroid grown in basement membrane extract (BME) monitored at different time points; C: HPAF-II 3D-spheroids grown in the absence or presence of BME were stained using rhodamine-phalloidin to detect actin filaments. Invadopodia are evident boxed. Original magnification: 60 ×.