Epithelial-to-mesenchymal transition in pancreatic ductal adenocarcinoma: Characterization in a 3D-cell culture model

Figure 4 PL45 ultrastructure. A: Electron microscopy of two PL45 cells grown in 2D-monolayers that exhibit protrusions consistent with invadopodia (ip) originating from their surface and projecting toward the culture medium. N: Nucleus. Scale bar = 1 μm; B: Light microscopy of a semi-thin section showing a multilayered spheroid with 2 small groups of cells partially detached from the periphery of the spheroid (arrows). Scale bar = 40 μm; C: Electron microscopy of a thin section adjacent to the semi-thin one showing a group consisting of 3 cells joined to each other. The outlined areas are shown at greater enlargement in inserts C1 and C2; arrows indicate desmosomes. N: Nucleus. Scale bar = 5 μm. Inset scale bar = 0.25 μm.