Methylation of DAPK and THBS1 genes in esophageal gastric-type columnar metaplasia

Table 1 Sequences of specific primers used for determining gene methylation status

Primer name		Forward primer sequence (5'-3')	Reverse primer sequence (5'-3')	Product size (bp)	AT (°C)	No. of cycles
DAPK	M	GGATAGTCGGATCGAGTTAACGTC	CCCTCCCAAACGCCGA	98	60	35
	U	GGAGGATAGTTGGATTGAGTTAATGTT	CAAATCCCTCCCAAACACCAA	98	60	35
p14	M	GTGTTAAAGGGCGGCGTAGC	AAAACCCTCACTCGCGACGA	122	60	40
	U	TTTTTGGTGTTAAAGGGTGGTGTAGT	CACAAAAACCCTCACTCACAACAA	132	60	40
CDH1	M	TTAGGTTAGAGGGTTATCGCGT	TAACTAAAAATTCACCTACCGAC	115	53	35
	U	TAATTTTAGGTTAGAGGGTTATTGT	CACAACCAATCAACAACACA	97	57	35
THBS1	M	TGCGAGCGTTTTTTTAAATGC	TAAACTCGCAAACCAACTCG	74	62	40
	U	GTTTGGTTGTTGTTTATTGGTTG	CCTAAACTCACAAACCAACTCA	115	62	40

M: Methylated sequence; U: Unmethylated sequence; AT: Annealing temperature.