Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

Figure 4 AMPK activation is required for MnSOD expression in HepG2. 215 cells. A: Cell lysates were subjected to Western blot analysis. The protein levels of AMPK and p-AMPK were detected; B: Cells were transfected with AMPK siRNAs (siAMPK) or a nonspecific control siRNA (siCTRL) for 6 d. The protein levels of AMPK and MnSOD were detected by Western blot analysis; C: Cells were subjected to 1 mM AMPK activator AICAR or inhibitor compound C for 6 d. The protein levels of AMPK, p-AMPK and MnSOD were detected by Western blot analysis; D: Cells were transfected with HBx siRNAs (siHBx) or a nonspecific control siRNA (siCTRL) for 6 d. The protein levels of AMPK and MnSOD were detected by Western blot analysis.