Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

Figure 2 Decreased mitochondrial superoxide production is accompanied by increased MnSOD expression and activity in HepG2. 215 cells. A: Mitochondrial superoxide level was decreased in HepG2.215 cells. Cells were cultured in serum-free medium for 6 d. Mitochondrial superoxide anion formation was measured by flow cytometry and MitoSOX. Quantification of mitochondrial superoxide anion is shown as mean ± SD of triplicates. ^aP < 0.05 vs HepG2 cell group; B: Cell lysates were subjected to Western blot analysis. The protein level of MnSOD was detected; C: MnSOD activity was measured with a commercial SOD Assay Kit. Quantification of MnSOD activity is shown as mean ± SD of triplicates. ^aP < 0.05 vs HepG2 cells group.