Eukaryotic elongation factor-1α 2 knockdown inhibits hepatocarcinogenesis by suppressing PI3K/Akt/NF-κB signaling

Figure 8 Overexpression of eEF1A2 promotes the proliferation, migration and invasion of SK-HEP-1 cells. SK-HEP-1 cells were infected with the negative lentivirus (NC), without lentivirus transfection (CON), or lentivirus-based eEF1A2 overexpression (OE). A: qRT–PCR; B: Western blot analysis confirmed the expression of eEF1A2 in the SK-HEP-1 cells; C: eEF1A2 promoted the proliferation of SK-HEP-1 cells in MTT assay; D: Cell cycle distribution detected by DNA ploidy analysis; E): Percentages of SK-HEP-1 cells in the G0/G1, S and G2/M phases; F: Migration; G: Metastasis in a Transwell chamber invasion assay. Data are mean ± SD (n = 3). ^aP < 0.05, ^bP < 0.01 vs NC and CON.