Eukaryotic elongation factor-1α 2 knockdown inhibits hepatocarcinogenesis by suppressing PI3K/Akt/NF-κB signaling

Figure 1 Relative eEF1A2 mRNA expression levels in hepatocellular carcinoma tissue samples and cells. GAPDH was used as an internal control. Expression data were obtained as 2^-ΔΔCT or -ΔΔCT relative to normal liver tissue values. A: Relative eEF1A2 mRNA expression levels in HCC tissue samples analyzed by the -ΔΔCT method. Normal liver tissue specimens (n = 20); matched liver tissue samples (n = 62); HCC tissue specimens (n = 62); B: Relative eEF1A2 mRNA expression levels in SK-HEP-1, HepG2 and BEL-7402 cells analyzed by 2^-ΔΔCT method (n = 3); C: eEF1A2 protein levels in matched liver tissue and HCC tissue specimens from a HCC patient, and normal liver tissue samples were analyzed by immunohistochemistry (SP × 200). Data are mean ± SD. ^bP < 0.01 vs Normal liver tissue and Matched liver tissue samples. ^dP < 0.01 vs SK-HEP-1 and HepG2.