Thymoquinone inhibits proliferation in gastric cancer via the STAT3 pathway in vivo and in vitro

Figure 2 Thymoquinone suppresses phospho-JAK2 and phospho-Src levels, but not STAT5 levels. A: HGC27 cells (1 × 10⁶/mL) were treated with the indicated concentrations of thymoquinone (TQ) for 24 h, and western blotting was performed; B: HGC27 cells (1 × 10⁶/mL) were treated with 50 μmol/L TQ for the indicated times, and western blotting was performed. The same blots were stripped and reprobed with the STAT5 antibody to verify equal protein loading; C: TQ suppressed phospho-JAK2 levels in a concentration -dependent manner. HGC27 cells (1 × 10⁶/mL) were treated with the indicated concentrations of TQ for 24 h, and Western blotting was performed. The same blots were stripped and reprobed with the JAK2 antibody to verify equal protein loading; D: TQ suppressed phospho-Src levels in a concentration -dependent manner. HGC27 cells (1 × 10⁶/mL) were treated with the indicated concentrations of TQ for 24 h, and Western blotting was performed. The same blots were stripped and reprobed with the Src antibody to verify equal protein loading. JAK: Janus-activated kinase.