Recombinant adenovirus containing hyper-interleukin-6 and hepatocyte growth factor ameliorates acute-on-chronic liver failure in rats

Figure 3 Effect of pDC315-3FLAG-sv40-HGF-IRES-Hyper-IL-6-EGFP on Ki67 proliferation index (immunohistochemistry, original magnification × 200) compared to those of pDC315-3FLAG-sv40-HGF-EGFP or pDC315-3FLAG-sv40-Hyper-IL-6-EGFP and quantification for Ki67⁺ hepatocytes versus total counted cells for all types of treated liver. ^aP < 0.05 vs Ad-GFP treated, ^bP < 0.01 vs control, ^cP < 0.05 vs Ad-HIL-6 treated, ^eP < 0.05 vs Ad-HGF treated. The results are expressed as the mean ± SEM, n = 8. Arrow is a Ki67⁺ cell. Control: Normal liver; model: ACLF liver; Ad-GFP: pDC315-3FLAG-sv40-EGFP; Ad-HIL-6: pDC315-3FLAG-sv40-Hyper-IL-6-EGFP; Ad-HGF: pDC315-3FLAG-sv40-HGF-EGFP; and Ad-HGF-HIL-6: pDC315-3FLAG-sv40-HGF-IRES-Hyper-IL-6-EGFP; A: Control at 24 h; B: Model at 24 h; C: Ad-GFP at 24 h; D: Ad-HIL-6 at 24 h; E: Ad-HGF at 24 h; F: Ad-HGF-HIL-6 at 24 h; G: Control at 48 h; H: Model at 48 h; I: Ad-GFP at 48 h; J: Ad-HIL-6 at 48 h; K: Ad-HGF at 48 h; L:Ad-HGF-HIL-6 at 48 h.