Recombinant adenovirus containing hyper-interleukin-6 and hepatocyte growth factor ameliorates acute-on-chronic liver failure in rats

Figure 1 Effect of pDC315-3FLAG-sv40-HGF-IRES-Hyper-IL-6-EGFP on the hepatic enzyme, prothrombin time, inflammatory cytokines, serum endotoxin and high mobility group box-1 in a rat acute-on-chronic liver failure model compared to those of pDC315-3FLAG-sv40-HGF-EGFP or pDC315-3FLAG-sv40-Hyper-IL-6-EGFP. A: Alanine transaminase (ALT); B: Prothrombin time (PT); C: Tumour necrosis factor-α (TNF-α); D: Interferon-γ (IFN-γ); E: Endotoxin; F: High mobility group box-1 (HMGB1). ^aP < 0.05 vs Ad-GFP treated, ^bP < 0.01 vs control, ^cP < 0.05 vs Ad-HIL-6 treated, ^eP < 0.05 vs Ad-HGF treated. The results are expressed as the mean ± SD, n = 6. Control: Normal liver; model: ACLF liver; Ad-GFP: pDC315-3FLAG-sv40-EGFP; Ad-HIL-6: pDC315-3FLAG-sv40-Hyper-IL-6-EGFP; Ad-HGF: pDC315-3FLAG-sv40-HGF-EGFP; Ad-HGF-HIL-6: pDC315-3FLAG-sv40-HGF-IRES-Hyper-IL-6-EGFP.