Combined mesenchymal stem cell transplantation and interleukin-1 receptor antagonism after partial hepatectomy

Figure 1 Characterization of mesenchymal stem cells (MSCs). MSCs transfected with a lentiviral vector encoding green fluorescent protein (GFP) were cultured for 3 d in vitro and observed by A: Light and B: Fluorescent microscopy (magnification × 200, scale bar = 500 μm); C: Flow cytometry of MSCs revealed > 97% expressed GFP after propagation; D: Surface markers of the cultured MSCs were identified by flow cytometry: > 90% of GFP-MSCs were positive for CD29 (upper right), CD44 (lower left) and CD90 (lower right); isotypic antibodies served as the control (upper left).