Functional analysis and drug response to zinc and D-penicillamine in stable ATP7B mutant hepatic cell lines

Figure 2 ATP7B mRNA and protein expression in mutant cell lines. A: Relative ATP7B mRNA expression in mutant cell lines was determined. The expression was normalized to GAPDH housekeeping gene. Gene expression as factor fold change relative to KO cells expressing wild type was calculated; B and C: Representative Western blots showing ATP7B protein at 37 °C and 30 °C, respectively.