Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease

Figure 7 Chronic alcohol induces serum AFP and cellular proliferation. A: Fold changes in serum AFP levels at week 8 and week 15; B: Liver AFP mRNA levels at week 15; C: Representative immunostaining images for proliferating cell nuclear antigen (PCNA) at week 15 (magnification × 400). Arrows point to positive staining; D: PCNA positive nuclei were scored in at least 3 different microscopic fields for each mouse and plotted as bar graph on the right. (n≥ 5 mice per group). In graphs, values are given as mean ± SD, Dunnett’s multiple comparison were used to compare the means of multiple groups; ^aP < 0.05. NS: Not significant; DEN: Diethyl nitrosamine; AFP: α-fetoprotein.