Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease

Figure 6 Chronic alcohol expedites hepatocellular carcinoma development. A: T2-weighted MRI of liver in coronal section at week 13. Arrows denote intrahepatic cysts. B: Representative HE stained intrahepatic cysts observed in all DEN injected mice (100X magnification). Note the higher number of cysts in alcohol + DEN mice. C: Quantification of cyst area using ImageJ. D: Representative HE stained liver sections from all treatment groups (magnification × 100) at week 15. The hepatic hyperplasia (encircled in black dotted line) was exclusively observed in alcohol + DEN mice. In graph (C), values are given as mean ± SD, Dunnett’s multiple comparison were used to compare the means of multiple groups; ^aP < 0.05, pair fed vs alcohol fed. ND: Not detected; DEN: Diethyl nitrosamine.