Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease

Figure 4 Chronic alcohol synergizes with diethyl nitrosamine in hepatocellular carcinoma development. A: Representative Oil Red O staining of hepatic sections (200 × magnification) from all experimental groups at 15 wk; B: Bar graph shows percent Oil Red O stained area in groups that was quantified using ImageJ software. For quantification, at least 3 different microscope fields were scored for each mouse (n≥ 5 mice per group); C: Liver triglycerides were estimated at week 15; D: Representative Sirius Red staining of hepatic sections (magnification × 100) from all experimental groups at 15 wk. E: For quantification of fibrosis, at least 3 different microscope fields at 10x magnification were scored for each mouse (n≥ 5 mice per group). Bar graph shows percent Sirius Red positive area quantified using ImageJ. In graphs, values are given as mean ± SD, Dunnett’s multiple comparison were used to compare the means of multiple groups; ^aP < 0.05. NS: Not significant; DEN: Diethyl nitrosamine.