Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease

Figure 3 Alcoholic hepatitis induces neutrophil infiltration. A: Liver mRNA levels of neutrophil markers, Ly6G, MPO and E-selectin; B: Representative myeloperoxidase (MPO) immunohistochemistry of hepatic sections (magnification × 200) from all experimental groups at 15 wk. Black arrows point to the positive staining; C: MPO positive cells were scored in at least 3 different microscopic fields for each mouse and plotted as bar graph. (n≥ 5 mice per group). In graphs, values are given as mean ± SD, Dunnett’s multiple comparison were used to compare the means of multiple groups; ^aP < 0.05. NS: Not significant; DEN: Diethyl nitrosamine.