CacyBP/SIP nuclear translocation regulates p27Kip1 stability in gastric cancer cells

Figure 7 Immunofluorescent localization of calcyclin binding protein/Siah-1 (1-72) in SGC7901-calcyclin binding protein/Siah-1si cells. SGC7901-CacyBP/SIPsi cells were transfected with pEGFP/C1 vector, producing either GFP-tagged CacyBP/SIP or CacyBP/SIP (1-72) lacking the 155 central and C-terminal amino acids. Cells transfected with GFP-tagged pEGFP/C1 lacking a cDNA insert served as controls. Transfectants were treated or untreated with gastrin (10^-8 mol/L), and EGFP fluorescence was analyzed under a confocal laser microscope. A, C, E: Unstimulated cells; B, D, F: Cells 8 h after gastrin stimulation.