Mir-30d increases intracellular survival of Helicobacter pylori through inhibition of autophagy pathway

Figure 4 Multiple core proteins in the autophagy pathway are direct targets of mir-30d in gastric epithelial cells. A: Luciferase reporter assay with plasmids bearing wild type or mutant 3′UTR binding sites of mir-30d in AGS cells with mir-30d mimic or control oligos. Luciferase activity of mir-30d mimic transfected cells was normalized to control mimic transfected cells. Results are shown as mean ± SD, ^aP < 0.05 vs control; B: mRNA levels of autophagy related (ATG)2B, ATG5, ATG12, beclin 1 (BECN1), and BNip3-like protein (BNIP3L) in both cell lines with mir-30d mimic or control mimic transfection. Results are shown as mean ± SD, ^aP < 0.05 vs control; C: ATG2B, ATG5, ATG12, BECN1, and BNIP3L protein levels in both cell lines with mir-30d mimic or control mimic transfection; D: The mRNA levels of ATG2B, ATG5, ATG12, BECN1, and BNIP3L in two cell lines with or without mir-30d inhibitor transfection. Results are shown as mean ± SD, ^aP < 0.05 vs control.