Auphen and dibutyryl cAMP suppress growth of hepatocellular carcinoma by regulating expression of aquaporins 3 and 9 in vivo

Figure 7 Effects of Auphen and dbcAMP on hepatocellular carcinoma in vivo. A: mRNA levels of α-fetoprotein (AFP) in tumors from nude mice; B: AFP concentration in blood of control (NS), Auphen-treated (Auphen), and dbcAMP-treated (dbcAMP) groups. All data represent the mean ± SD (n = 3). ^bP < 0.01 vs the control; ^eP < 0.001 vs the control; C: Testing of apoptosis by TUNEL assay in tumor samples of nude mice in different treated groups (magnification, × 400). The nuclei of positive cells were stained brown; D: Tumor samples from nude mice in different treated groups (HE, magnification, × 400). Pathological traits of positive cells were revealed as karyopyknosis, and had a cytoplasmic red color.