Auphen and dibutyryl cAMP suppress growth of hepatocellular carcinoma by regulating expression of aquaporins 3 and 9 in vivo

Figure 5 Effects of Auphen and dibutyryl cAMP on AQP3 and AQP9 expression. A: mRNA levels of AQP3 in tumors from nude mice; B: mRNA levels of AQP9 in tumors from nude mice; C: Protein levels of AQP3 and AQP9 in tumors from nude mice; D: Immunohistochemical analysis of a subcutaneous tumor: a: Analysis of AQP3 expression in the control group; b: Analysis of AQP3 expression in the Auphen group; c: Analysis of AQP9 expression in the control group; d: Analysis of AQP9 expression in the dbcAMP group. (magnification × 200, bar = 50 μm). All data represent the mean ± SD (n = 3). ^bP < 0.01 vs the control; ^eP < 0.001 vs the control. dbcAMP: Dibutyryl cAMP.