Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer’s patches

Figure 3 Assay of Treg cells. A: Representative flow plots of CD4⁺CD25⁺Foxp3⁺ T cells. Histograms show the distribution of immunofluorescence labeling intensity of CD4, CD25 and Foxp3 expression in each group. Ordinate indicates cell counts; abscissa represents fluorescent intensity. B: Levels of CD4⁺CD25⁺Foxp3⁺ T cells. Data are mean ± SEM (n = 8). ^aP < 0.05 vs normal group; ^bP < 0.01, ^fP < 0.0001 vs TNBS group. TNBS: 2,4,6-trinitrobenzene sulfonic acid; APS: Astragalus polysaccharide; MES: Mesalazine.