Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas

doi:10.3748/wjg.v21.i9.2629

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 21, Issue 9

This Article

Academic Content and Language Evaluation of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (7801)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Figures (1-2) series, Tables (1-3) series.

Item

Count

PDF

565

WORD

217

HTML

4721

Figures (1-2)

241

Tables (1-3)

264

Sum=6008

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

606

Download

1187

Sum=1793

Mar 7, 2015 (publication date) through Jan 2, 2025

Times Cited of This Article

Times Cited (50)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastroenterol. Mar 7, 2015; 21(9): 2629-2637
Published online Mar 7, 2015. doi: 10.3748/wjg.v21.i9.2629

Table 1 Summary of primer sequences and annealing temperatures used for methylation-specific polymerase assays

Gene	Primer	Sequence (5'-3')	Annealing temperature (°C)	Ref.
GATA5	MF	TTAGAAATCGAGGAAATCGC	54
	MR	GTAAACCCCCTCGTTACGTA
	UF	TGTTTAGAAATTGAGGAAATTGT	48
	UR	CCCATAAACCCCCTCATTACATA
SFRP2	MF	TTTTTGTAGGGGCGTTTTTATAAC	58	[21]
	MR	TATCGATATACTCCCCAATACCG
	UF	AGATTTTTGTAGGGGTGTTTTTATAAT	54
	UR	ACCTATCAATATACTCCCCAATACCA
ITGA4	MF	TAGAGTTATTTCGCGTTTTGCG	56	[22]
	MR	CTTCGAATACTCGCGCTACTT
	UF	GTTTAGAGTTATTTTGTGTTTTGTG	50
	UR	AAAACTTCAAATACTCACACTACT

M: Methylated; U: Unmethylated; F: Forward; R: Reverse.

Citation: Zhang X, Song YF, Lu HN, Wang DP, Zhang XS, Huang SL, Sun BL, Huang ZG. Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas. World J Gastroenterol 2015; 21(9): 2629-2637
URL: https://www.wjgnet.com/1007-9327/full/v21/i9/2629.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i9.2629