Silencing profilin-1 inhibits gastric cancer progression via integrin β1/focal adhesion kinase pathway modulation

Figure 6 The effect of fibronectin is ameliorated after profilin-1 silencing. A: SGC-7901 cells with and without profilin-1 (PFN1) silencing were seeded onto media with or without fibronectin (FN) (10 μg/mL) for 24 h. The expression of phosphorylated focal adhesion kinase (FAK) was evaluated by Western blot; B: SGC-7901 cells with and without PFN1 silencing were seeded on media with or without FN (10 μg/mL) at the indicated time points. Cell proliferation levels were measured using the cell counting kit-8 at 12, 24 and 48 h. The optical density (A) represents the proliferative characteristics of the treated cells; C: SGC-7901 cells with and without PFN1 silencing were suspended in media with or without FN (10 μg/mL) and seeded onto transwell plates. The cell migration was assessed 24 h later. For the invasion assays, the cells were seeded onto BD Matrigel invasion chambers. ^aP < 0.05, ^bP < 0.01 vs other groups. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Si: Silencing.