Silencing profilin-1 inhibits gastric cancer progression via integrin β1/focal adhesion kinase pathway modulation

Figure 5 Profilin-1 silencing regulates the expression of integrin β1 and its downstream molecular targets. A and B: Expression of integrin β1 protein and mRNA was detected by Western blot and quantitative real-time polymerase chain reaction analyses, respectively; C: The effect of profilin-1 (PFN1) knockdown on its downstream targets in the focal adhesion kinase (FAK) signaling pathway. The quantity of PFN1 was normalized against that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the phosphorylation of other proteins was normalized against the respective total protein. All results were obtained from three independent experiments. ^aP < 0.05, ^bP < 0.01 vs other groups. ERK: Extracellular-regulated kinase; mTOR: Mammalian target of rapamycin; PI3K: Phosphatidylinositol 3-kinase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Negative control; Si: Silencing.