Silencing profilin-1 inhibits gastric cancer progression via integrin β1/focal adhesion kinase pathway modulation

Figure 3 Profilin-1 knockdown inhibits tumor growth. A: Expression of profilin-1 (PFN1) was assessed in the PFN1-transfected cells, the untreated control and the negative control (NC)-transfectants by Western blot; B: Cell proliferation levels were measured using the cell counting kit-8 on 1, 2, 3, 4 and 5 d. The optical density (A) represents the proliferative characteristics of the treated cells; C: PFN1 silencing resulted in significantly lower colony-forming efficiency; D: Cell-cycle distribution was analyzed by flow cytometry on SGC-7901 cells. The results obtained from three independent experiments are shown. ^aP < 0.05, ^bP < 0.01 vs other groups. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Si: Silencing.