Copyright
©The Author(s) 2015.
World J Gastroenterol. Feb 28, 2015; 21(8): 2343-2351
Published online Feb 28, 2015. doi: 10.3748/wjg.v21.i8.2343
Published online Feb 28, 2015. doi: 10.3748/wjg.v21.i8.2343
Table 3 Polymerase chain reaction reaction system setup
| Polymerase chain reaction reaction system | Volume (μL) |
| LongAmp® Taq DNA polymerase | 1 |
| LongAmp Taq reaction buffer | 5 |
| Deoxynucleotide solution mix | 3 |
| Dimethyl sulfoxide | 1.25 |
| Forward primer (10 μmol/L) | 1 |
| Reverse primer (10 μmol/L) | 1 |
| DNA template | 1 |
| H2O | 11.75 |
| Total volume | 25 |
- Citation: Jin S, Cui K, Sun ZQ, Shen YY, Li P, Wang ZD, Li FF, Gong KN, Li S. Screening analysis of candidate gene mutations in a kindred with polycystic liver disease. World J Gastroenterol 2015; 21(8): 2343-2351
- URL: https://www.wjgnet.com/1007-9327/full/v21/i8/2343.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i8.2343