Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Feb 28, 2015; 21(8): 2343-2351
Published online Feb 28, 2015. doi: 10.3748/wjg.v21.i8.2343
Table 2 Primers for sequencing of PKD1 exons
Primer nameSequenceGenomic position (starting from the 5’ end)Distance to exon-intron junctionExons covered
PKD1-exon1-F5-GCGTCGCTCAGCAGCAGGT-32185830-140 Ex11
PKD1-exon1-R5-GCCCGCGTCCTGCTTCCC-3218538393 Ex1
PKD1-exon2-3-F5-GGGATGCTGGCAATGTGTGGGAT-32169468-89 Ex22-3
PKD1-exon2-3-R5-GGACCAACTGGGAGGGCAGAA-3216903877 Ex3
PKD1-exon4-F5-GGCGGTGCTGTCAGGGTG-32168948-102 Ex44
PKD1-exon4-R5-CCAGAGAGGCCTTCCTGAGC-3216858295 Ex4
PKD1-exon5A-F5-GAACAGCATGGGAGCCTGTGAGT-32168561-98 Ex55
PKD1-exon5A-R5-AGCCGGCCCAGCGGCATC-32168033Within an exon
PKD1-exon5B-F5-GCCTGTCCCTCTGCTCCG-32168262Within an exon5
PKD1-exon5B-R5-GTGTCAACGGTCAGTGTGGGC-3216769696 Ex5
PKD1-exon6-F5-GTGTCTGCTGCCCACTCCC-32167787-124 Ex66
PKD1-exon6-R5-CTCCTTCCTCCTGAGACTCCC-3216739793 Ex6
PKD1-exon7-F5-GCTGCTGTGAGGGTGGGAGGA-32167120-66 Ex77
PKD1-exon7-R5-TCCACCGCGGGCGCTCGGCA-3216676371 Ex7
PKD1-exon8-F5-CTGGGCTGAGGAGGAGGG-32166760-115 Ex88
PKD1-exon8-R5-GGGCACAAGCAACATTAAGGCCC-32166413117 Ex8
PKD1-exon9-F5-CCTCTTCCTGGGAAGTTCGGGT-32166206-87 Ex99
PKD1-exon9-R5-ACTCTGGTGGCCACAGGACCA-3216589598 Ex9
PKD1-exon10-F5-GCAGGCAGTTGGGCATCTCTG-32165697-71 Ex1010
PKD1-exon10-R5-GACCCTGGGCAGCAGACAG-3216530970 Ex10
PKD1-exon11A-F5-GTGTGGCTGACGAAGCGGG-32165035-109 Ex1111
PKD1-exon11A-R5-CCGTGGCGTTGGCACCAG-32164455Within an exon
PKD1-exon11B-F5-CGCTATGAGGTCCGGGCAG-32164641Within an exon11
PKD1-exon11B-R5-CCCTCACTGGGAAGCCAGG-3216407793 Ex11
PKD1-exon12-F5-GGACTCTCCAGCCCGACG-32163387-94 Ex1212
PKD1-exon12-R5-GCAGAGGTGAAGGTGGAGC-3216308874 Ex12
PKD1-exon13-14-F5-GTGGAGGGAGGGACGCCAA-32163037-73 Ex1313-14
PKD1-exon13-14-R5-GTCACAGTGAGGGCTGTTGGG-32162230111 Ex14
PKD1-exon15A-F5-TTCTGCCGAGCGGGTGGG-32161938-64 Ex1515
PKD1-exon15A-R5-CATGTCGAAGGTCCACGTGATGT-32161427Within an exon
PKD1-exon15B-F5-GACATGAGCCTGGCCGTGG-32161516Within an exon15
PKD1-exon15B-R5-CCACCTCTGGCTCCACGCA-32161027Within an exon
PKD1-exon15C-F5-CACGCGGAGCGGCACGTT-32161121Within an exon15
PKD1-exon15C-R5-GGTGACCTCCGGACCCTC-32160626Within an exon
PKD1-exon15D-F5-TCTGCTGTGGGCCGTGGG-32160706Within an exon15
PKD1-exon15D-R5-CTGTACCGTGTGGTTGGTGGG-32160215Within an exon
PKD1-exon15E-F5-ACAGCATCTTCGTCTATGTCCTG-32160303Within an exon15
PKD1-exon15E-R5-GGTTCCCTGCCGTCATGGTG-32159812Within an exon
PKD1-exon15F-F5-GGGCTGAGCTGGGAGACCT-32159899Within an exon15
PKD1-exon15F-R5-GACAGCTGAGCCGGCAGC-32159417Within an exon
PKD1-exon15G-F5-CTGTGGGCCAGCAGCAAGGT-32159494Within an exon15
PKD1-exon15G-R5-CGTGCGGTTCTCACTGCCCA-32159012Within an exon
PKD1-exon15H-F5-GACGTCACCTACACGCCCG-32159095Within an exon15
PKD1-exon15H-R5-CCTCCCAGCGGTACTCAGTCT-32158603Within an exon
PKD1-exon15I-F5-GATGCGGCGATCACAGCGCA-32158688Within an exon15
PKD1-exon15I-R5-GGCCAGCCCTGGTGGCAA-3215816489 Ex15
PKD1-exon16-F5-GGCCCGTCCTCAGTGCCT-32158167-134 Ex1616
PKD1-exon16-R5-GCGGCCTCCACCAGCACTA-3215779094 Ex16
PKD1-exon17-18-F5-GAAACCTGGAGTTTGGGAGCAGC-32157047-98 Ex1717-18
PKD1-exon17-18-R5-TGACGTCACAGAGTCGGG-3215634455 Ex18
PKD1-exon19-20-F5-GCACGGGTGAGTGCAGGC-32156404-99 Ex1919-20
PKD1-exon19-20-R5-CCGGGATGAGCCCTCTGCAA-3215576898 Ex20
PKD1-exon21-F5-AGTCGTGGGCATCTGCTGGC-32155550-75 Ex2121
PKD1-exon21-R5-CAAGCTGCCCGTCTGCCCT-3215524083 Ex21
PKD1-exon22-F5-CAGGTGAGGACCCGTGTAGAGA-32154725-82 Ex2222
PKD1-exon22-R5-GGGAGGAGGGAGGCAGAG-3215443168 Ex22
PKD1-exon23A-F5-GCACCTCGCTCTCTGCCC-32154024-128 Ex2323
PKD1-exon23A-R5-GCCACCTTGGTGGAGACGG-32153512Within an exon
PKD1-exon23B-F5-GGCTGCCACTTCTCCATCCC-32153651Within an exon23
PKD1-exon23B-R5-GACACCCATGGAAGCCCTACG-3215318384 Ex23
PKD1-exon24-F5-CGTGGCAGAGGGTGGGCT-32153064-93 Ex2424
PKD1-exon24-R5-CTCGCTGCCTGCCGTCCC-3215272194 Ex24
PKD1-exon25-26-F5-GGCTCTGAGACTGCGACATCCAA-32152702-68 Ex2525-26
PKD1-exon25-26-R5-CTTGTTCTGACGCCTGCGACG-3215196498 Ex26
PKD1-exon27-28-F5-GCTGAGATGACTTGCCTGGGATG-32150644-77 Ex2727-28
PKD1-exon27-28-R5-ACTGCAGGAGGCCACGGG-3215009473 Ex28
PKD1-exon29-30-F5-CTCCGTGGGAGGTTGGGCA-32150142-70 Ex2929-30
PKD1-exon29-30-R5-CGCCTTTCCCTCTGGCTGC-32149542103 Ex30
PKD1-exon31-32F5-CGGGCTCTGTCCTGTCTGC-32148079-94 Ex3131-32
PKD1-exon31-32R5-CCCAGCAAGGACACGCAGC-3214764287 Ex32
PKD1-exon33-34-F5-GGAAGCCCAGGGTGTCCGT-32147595-91 Ex3333-34
PKD1-exon33-34-R5-CAGCCCTGCCCTGGCACC-32147034115 Ex34
PKD1-exon35-F5-CAAGAGGCTCAAGAAACTGCCCG-32144309-98 Ex3535-36
PKD1-exon36-R5-GAGAAGTACAGGGCTTCCAGCAA-3214371498 Ex36
PKD1-exon37-F5-CTCGGCTGGGAGCCACTG-32143832-93 Ex3737
PKD1-exon37-R5-GCCTTCTGAGGTGAGGAAAGGG-32143439106 Ex37
PKD1-exon38-F5-CCACACCTGCCGCAGCCAT-32143190-96 Ex3838
PKD1-exon38-R5-CAAAGGTATCTACACATGTCCAC-3214285699 Ex38
PKD1-exon39-F5-GCCAGCAGGGCAGTGGGA-32142698-105 Ex3939
PKD1-exon39-R5-CAGCTAGGGAGCAGGGCTGA-3214238596 Ex39
PKD1-exon40-F5-GTGGCGCCGAACCAGAGC-32142289-100 Ex4040-41
PKD1-exon41-R5-GGGCTGTGGAAGCCGCCTA-32141678104 Ex41
PKD1-exon42-F5-CCTCAGCCACGCCTGCACT-32141678-80 Ex4242
PKD1-exon42-R5-GGGTGAGACGCTGCCGGG-3214133886 Ex42
PKD1-exon43-F5-CAGCGTCCCTCCCGCCCT-32141213-38 Ex4343-44
PKD1-exon44-R5-CAGGAAGACACGAGCTGCGG-3214057897 Ex44
PKD1-exon45-F5-GCTGGCCATCCTGGTAGGTGA-32140687-96 Ex4545
PKD1-exon45-R5-GGACTTTGTGGCGGAACTGGG-32140180106 Ex45
PKD1-exon46-F5-GGAGAGGGACACGCCCTG-32140264-69 Ex4646
PKD1-exon46-R5-ATTCTGCCTGGCCCTCGGCCTT-32139635Within 3UTR