Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing

Figure 1 Generation of PICM-19-CSCs. A: Feeder-independent PICM-19 cells were grown in 10% DMEM/Medium 199 on Matrigel in 24-well plates (see Materials and Methods). They were transfected with pUNO1-MYC-IRES-Luc plasmid and tested for luciferase expression using luciferin as substrate; B: Untransfected PICM-19 cells did not show bioluminescence (left panel), whereas transfected cells expressed luciferase (right panel); C: Detection of MYC protein in PICM-19-CSCs by Western blotting using the anti-human mouse c-MYC monoclonal antibody. As expected, PICM-19-CSCs, but not the untransfected PICM-19 stem cells (control) expressed MYC.